anti p25 p35  (Cell Signaling Technology Inc)

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: HG-induced TrkA phosphorylation is associated with podocyte inflammation and injury. (A–D) Representative western blots show the levels of (A) TrkA and phospho-TrkA (Tyr490); (B) ERK, phospho-ERK and EGR1; (C) p35 and CDK5; and (D) IL-1β and TNF-α in MPC5 cells from the LG, HG, and LG + Man groups at 24 h (E) Representative immunofluorescence double staining shows the expression of phospho-TrkA (Tyr490) and synaptopodin in MPC5 cells from the LG, HG, and LG + Man groups. ****p < 0.0001. p < 0.05 was considered statistically significant; ns indicates not significant.

Article Snippet: Antibodies against TrkA (Cat. No. 2505), Cdk5 (Cat. No. 2506), and p35/25 (Cat. No. 2680) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Phospho-proteomics, Western Blot, Immunofluorescence, Double Staining, Expressing

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibitor suppresses p-TrkA-mediated upregulation of ERK, EGR1, and p35 in the MPC5 cells grown under HG conditions. Representative western blots show the levels of (A) TrkA and phospho-TrkA (Tyr490), (C) ERK, phospho-ERK, and EGR1, and (E) CDK5 and p35 in the LG, HG+Vehicle (0.1% DMSO), HG + Inh-TrkA, HG + empty vector, HG + TrkA OE, and HG + TrkA OE + PD98059 groups. Representative western blots show the levels of (B) TrkA and phospho-TrkA (Tyr490), (D) ERK, phospho-ERK, and EGR1, and (F) CDK5 and p35 in the LG, HG+si-NC, HG+si-TrkA, HG + TrkA OE+Ctrl, and HG + TrkA OE + GW441756 groups. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001. p < 0.05 was considered statistically significant.

Article Snippet: Antibodies against TrkA (Cat. No. 2505), Cdk5 (Cat. No. 2506), and p35/25 (Cat. No. 2680) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Western Blot, Plasmid Preparation

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: TrkA inhibition reduces the levels of p-TrkA, p-ERK, EGR1, p35, and CDK5 in the kidneys of db/db mice. (A–C) Representative western blots show the protein expression levels of (A) TrkA and phospho-TrkA (Tyr490), (B) ERK, p-ERK and EGR1, and (C) p35 and CDK5 in the kidney samples from the control, db/db, Inh-NC, and Inh-TrkA groups of mice. (D) Representative IF images show the expression of Synaptopodin (red) in the glomeruli of control, db/db, Inh-NC, and Inh-TrkA groups of mice (400X). ***p < 0.001. p < 0.05 was considered statistically significant; ns indicates not significant.

Article Snippet: Antibodies against TrkA (Cat. No. 2505), Cdk5 (Cat. No. 2506), and p35/25 (Cat. No. 2680) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Inhibition, Western Blot, Expressing, Control

Journal: Frontiers in Endocrinology

Article Title: Unveiling the TrkA-p35/CDK5 axis: a novel therapeutic target in diabetic kidney disease

doi: 10.3389/fendo.2026.1791283

Figure Lengend Snippet: Schematic diagram shows the TrkA/ERK/p35/CDK5 regulatory axis and the site of action for the TrkA inhibitor GW441756 in the murine renal podocytes.

Article Snippet: Antibodies against TrkA (Cat. No. 2505), Cdk5 (Cat. No. 2506), and p35/25 (Cat. No. 2680) were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques:

Journal: Communications Biology

Article Title: Tau is necessary for Pseudomonas aeruginosa -induced blood-brain barrier dysfunction

doi: 10.1038/s42003-026-09703-x

Figure Lengend Snippet: Representative western blots and quantification 24 h post-infection of ( a ) Gsk3β-p216 (active form) ( t = 0.7274; p = 0.2472; n = 4/group; 95% CI [−0.1879, 0.3468]; d = 0.5112) ( b ) p35 ( t = 2.6090; p = 0.0402; n = 4/group; 95% CI [−0.6799, −0.02177]; d = 1.8449) and Cdk5 ( t = 1.4770; p = 0.1902; n = 4/group; 95% CI [−0.04456, 0.1802]; d = 1.0474) ( c ) pAkt/GAPD ( t = 5.8970; p = 0.0011; n = 4/group; 95% CI [−0.9844, −0.4070]; d = 4.1698), pAkt/Akt ( t = 6.5620; p = 0.0006; n = 4/group; 95% CI [−0.9859, −0.4504]; d = 4.6415) and Akt/GAPDH ( t = 0.2954; p = 0.7776; n = 4/group; 95% CI [−0.2753, 0.3509]; d = 0.2100). Representative western blots and quantification 48 h post-infection of ( d ) Gsk3β-p216 (active form) ( t = 0.2533; p = 0.4043; n = 5 saline, 3 PA103; 95% CI [−0.4672, 0.5750]; d = 0.1808), ( e ) p35 ( p = 0.0357; n = 5 saline, 3 PA103; 96.43% CI [−0.07233, −0.04665]; d = 2.6897) and Cdk5 ( t = 0.8005; p = 0.4539; n = 5 saline, 3 PA103; 95% CI [−0.03866, 0.07625]; d = 0.6215) ( f ) pAkt/GAPDH ( t = 6.9350; p = 0.0004; n = 5 saline, 3 PA103; 95% CI [−0.8849, −0.4233]; d = 5.1622), pAkt/Akt ( t = 16.0800; p < 0.0001; n = 5 saline, 3 PA103; 95% CI [−0.8136, −0.5987]; d = 10.5527) and Akt/GAPDH ( t = 1.8110; p = 0.1201; n = 5 saline, 3 PA103; 95% CI [−0.05736; 0.3840]; d = 1.2331). Data is shown as mean ± SEM with n = 3–5 mice per group. All data were normally distributed except e ; all data had equal variance; everything was two-tailed t-test except a , d were one-tailed t-test; p ≤ 0.05 considered significant. Open shapes indicate male mice. Filled shapes indicate female mice. Light blue circles represent saline mice. Dark blue squares represent PA103-infected mice.

Article Snippet: Rabbit anti-p35/25 (1:1000); Cell Signaling 2680 , Donkey anti-rabbit IgG HRP (1:1000); Invitrogen SA1-200.

Techniques: Western Blot, Infection, Saline, Two Tailed Test, One-tailed Test

Journal: Communications Biology

Article Title: Tau is necessary for Pseudomonas aeruginosa -induced blood-brain barrier dysfunction

doi: 10.1038/s42003-026-09703-x

Figure Lengend Snippet: Representative images and quantification of integrated density of IgG leakage from vessels (lectin) in the ( a ) hippocampus ( t = 1.4250; p = 0.1779; n = 6 saline, 9 PA103; 95% CI [−0.8428, 0.1730]; d = 0.6920) and ( b ) cortex ( t = 1.3190; p = 0.2368; n = 6 saline, 10 PA103; 95% CI [−1.049, 0.3180]; d = 0.7436). Representative images and quantification of integrated density of pericyte (CD13) coverage of vessels (lectin) in the ( c ) hippocampus ( t = 0.2003; p = 0.8479; n = 3 saline, 5 PA103; 95% CI [−8.214, 9.679]; d = 0.1514) and ( d ) and cortex ( t = 0.4651; p = 0.6583; n = 3 saline, 5 PA103; 95% CI [−6.694, 9.836]; d = 0.3009). Representative images and quantification of Iba1 integrated density in ( e ) hippocampus ( t = 1.776; p = 0.0305; n = 3 saline, 7 PA103; 95% CI [−0.0562, 0.4329]; d = 1.4961) and ( g ) cortex ( t = 1.360; p = 0.2068; n = 4 saline, 7 PA103; 95% CI [−0.1096, 0.4404]; d = 0.9322). Quantification of microglia morphology in the ( f ) hippocampus and ( h ) cortex. ( i ) Representative images with DAPI and quantification of integrated density of GFAP in the hippocampus ( t = 1.4810; p = 0.1822; n = 3 saline, 6 PA103; 95% CI [−0.6344, 0.1458]; d = 1.2220). j Western blots and quantification of tight junction proteins, ZO-1 ( t = 1.3980; p = 0.2027; n = 6 saline, 7 PA103; 95% CI [ − 0.6429, 2.551]; d = 0.7528), occludin ( t = 1.4700; p = 0.1722; n = 6/group; 95% CI [−0.1181, 0.5761]; d = 0.8487), and claudin-5 ( t = 0.8028; p = 0.4391; n = 6 saline, 7 PA103; 95% CI [−0.6787, 0.3159]; d = 0.4461) with loading control β-actin, in isolated capillaries 24 h post-infection. Representative western blots and quantification of ( k ) p35 ( t = 5.4060; p = 0.0017; n = 3 saline, 5 PA103; 95% CI [−0.2909, −0.1096]; d = 3.4518) and Cdk5 ( t = 1.8100; p = 0.1133; n = 3 saline, 6 PA103; 95% CI [−0.04279, 0.3219]; d = 1.3480) and ( l ) pAkt/GAPDH ( t = 4.6440; p = 0.0390; n = 3 saline, 6 PA103; 95% CI [−1.368, −0.08695]; d = 3.7408) pAkt/Akt ( t = 3.7940; p = 0.0584; n = 3 saline, 6 PA103; 95% CI [−1.490, 0.06076]; d = 3.0626) and Akt/GAPDH ( t = 0.8549; p = 0.4209; n = 3 saline, 6 PA103; 95% CI [−0.1913, 0.0897]; d = 0.6062) 24 h post-infection. Data is shown as mean ± SEM with n = 3–9 mice per group. All data were normally distributed; all data had normal variance except b , j , e , and l ; two-tailed t-test for all except f and h ; p ≤ 0.05 considered significant. Scale bar = 45 μm. Open shapes indicate male mice. Filled shapes indicate female mice. Light brown circles represent saline mice. Dark brown squares represent PA103-infected mice.

Article Snippet: Rabbit anti-p35/25 (1:1000); Cell Signaling 2680 , Donkey anti-rabbit IgG HRP (1:1000); Invitrogen SA1-200.

Techniques: Saline, Western Blot, Control, Isolation, Infection, Two Tailed Test